Phospho erk antibody cst

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August undefined, 2024

WebPhospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P) Immunohistochemistry analysis of ERK1/2 (pTpY185/187) showing staining in the … WebThe cells were labeled with ERK1 + ERK2 Antibody [ERK-7D8] (ab54230) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 ® Rabbit Anti-Mouse IgG Secondary Antibody at a dilution of 1/400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI.

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody …

Webphospho erk antibodies (Cell Signaling Technology Inc) Cell Signaling Technology Inc is a verified supplier Cell Signaling Technology Inc manufactures this product About News … WebAug 3, 2015 · Importantly, the two anti-phospho-ERK1/2 antibodies tested here displayed different selectivities for the presumed monophosphorylated ERK1 and ERK2 species: The monoclonal rabbit antibody... fisher and paykel repairs auckland

Detection and Differentiation of Threonine- and Tyrosine ... - Nature

WebMay 31, 2024 · Background We recently reported that STAT1 plays a tumor suppressor role, and ERK was inversely correlation with STAT1 expression in esophageal squamous cell carcinoma (ESCC). Here, we investigated the mechanism(s) that are responsible for the ERK regulates STAT1 in ESCC. Methods We performed the immunoprecipitation (IP) to detect … WebAn immobilized capture antibody specific for ERK1 / ERK2 binds both phosphorylated and unphosphorylated ERK1 / ERK2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format. Complete Your Research Human/Mouse/Rat Phospho-ERK1 (T202/Y204) DuoSet … Web(A) Western blot analysis of Akt (phsopho-S473 antibody) and Erk1/2 (phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) phosphorylation levels in retinas and (B) in the dorsolateral geniculate nucleus (dLGN) of the brain tissues lysates (8 weeks of chronic elevated IOP) and their densitometric quantitative analysis (* P < 0.05, ** P < 0.01, *** P < … fisher and paykel repair nz

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody …

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA

WebThe Phospho-ERK (Thr202/Tyr204) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the … WebUpon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. Flow Cytometry Panel Builder fisher and paykel reliability refrigeratorsWebPhospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P) Immunohistochemistry analysis of ERK1/2 (pTpY185/187) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). fisher and paykel repairs gold coast

"WebThe Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody. This antibody recognizes Human, Mouse, Rat, … " - Phospho erk antibody cst

Phospho erk antibody cst

WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase. … WebAnti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody Description Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) Host species Rabbit Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Human Predicted to work with: Mouse, Rat, Cow Immunogen

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WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 1049 publications. Validated for WB, WB, IF, F. Available in 2 sizes. … WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse …

WebMay 14, 2024 · After initiating T-cell signaling with the dimerizer, we pulsed the cell conjugates with light after 5 min for 3 min before returning them to the signaling-competent state. ... The resuspended pellet was then incubated with the phospho-ERK1/2 (Thr 202 /Tyr 204) antibody directly conjugated with Alexa Fluor 488 for 45 min at room temperature ... WebThis antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology. 36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

WebPhospho-ERK Assays Extracellular signal-related kinase (ERK1/2 or p42/44) is a kinase in the mitogen-activated protein kinase (MAPK) family and phosphorylation of ERK (p-ERK) … WebThis kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels, THUNDER Format: Half-area 96-well or low-volume 384-well plate Formulation: Eu-phospho-ERK (20 uL) Acc-phospho-ERK (80 uL) Eu-Total ERK (5 uL) Acc-Total ERK (20 uL) ERK control lysate (500 uL) 5X Lysis Buffer #1 (5 mL) 10X TR-FRET Detection buffer (250 uL) Gene ...

WebThe Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody. This antibody recognizes Human, Mouse, Rat, Bovine, C. elegans/Worm, Drosophila/Arthropod, Hamster, Non-Human Primate, Porcine, Zebrafish/Fish, and Other Mammalian antigen.

WebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody … canada post grimshaw albertaWebCyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a … canada post greely ontarioWebThe Phospho-Specific Mouse Anti-EGF Receptor pSer1047 ( VMA00746) and anti-total Rabbit Anti-EGF Receptor were incubated together. hFAB Rhodamine Anti-Tubulin was added with the fluorescent secondary antibodies. The fluorescent images were captured using the ChemiDoc MP Imaging System. 5 Phosphatase treatment canada post hanmer hoursWebPhospho Erk Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … canada post government ownedWebNov 20, 2024 · Phosphorus is a mineral the body needs to build strong bones and teeth. It is also important for nerve signaling and muscle contraction. This test is ordered to see how … fisher and paykel repairs canberraWebProduct Details Antibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: p44/42 MAPK; Extracellular signal-Regulated Kinase 1/2 … fisher and paykel repairs melbourneWebCell Signaling Technology's Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. canada post head office ontario